Karyotype and chromosome location of characteristic tandem repeats in the pufferfish <i>Tetraodon nigroviridis</i> - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cytogenetic and Genome Research Année : 2000

Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis

C. Ozouf-Costaz
  • Fonction : Auteur
H. Roest Crollius
  • Fonction : Auteur
C. Dasilva
  • Fonction : Auteur
O. Jaillon
L. Bouneau
  • Fonction : Auteur
C. Bonillo
  • Fonction : Auteur
J. Weissenbach
  • Fonction : Auteur
  • PersonId : 911249

Résumé

Karyotype analysis of Tetraodon nigroviridis, a pufferfish of the family Tetraodontidae with a small compact genome (385 Mb) which is currently being investigated in our laboratory, indicates that this species has 2n = 42 chromosomes. The small chromosome size (the largest pair measuring less than 3 μm) has complicated accurate chromosome pairing based on morphology alone. DAPI staining, however, provides a banding-like pattern. Because of quantitative variations of some heterochromatin classes, the chromosome formula can not be established precisely, but is estimated to include approximately 20 meta- or submetacentric chromosomes and 22 subtelocentric chromosomes. A centromeric satellite, telomeric repeats, and the major and minor rRNA clusters have been localized unequivocally by FISH. As a result, the 28S and 5S rDNA sequences can be used as chromosome-specific probes.
Fichier non déposé

Dates et versions

hal-04398482 , version 1 (16-01-2024)

Identifiants

Citer

C. Fischer, C. Ozouf-Costaz, H. Roest Crollius, C. Dasilva, O. Jaillon, et al.. Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis. Cytogenetic and Genome Research, 2000, 88 (1-2), pp.50-55. ⟨10.1159/000015484⟩. ⟨hal-04398482⟩
7 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More