Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 2024

Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples

Résumé

Tick-borne pathogens (TBPs) are often detected through classical molecular tools (PCR, nested PCR, real-time PCR), but these are limited in terms of the number of targeted pathogens due to the volume of DNA available for analysis. To solve this problem, in 2014 we developed a new high-throughput method based on real-time microfluidic PCRs that can detect 48 or 96 pathogens in 48 or 96 samples in a single run, such as ten species from the Borrelia burgdorferi sensu lato group. We then used this technique for large-scale epidemiological studies of TBPs in tick and animal samples on an international scale through numerous collaborative projects.
Fichier non déposé

Dates et versions

hal-04398431 , version 1 (16-01-2024)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Sara Moutailler, Clemence Galon. Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples. Humana. Borrelia burgdorferi, 2742, Springer US, pp.1-17, 2024, Methods in Molecular Biology, 978-1-0716-3560-5. ⟨10.1007/978-1-0716-3561-2_1⟩. ⟨hal-04398431⟩
20 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More