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Pré-Publication, Document De Travail Année : 2024

Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR)

1 UCC - University College Cork
2 MSU - Lomonosov Moscow State University
3 IBCh RAS - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
4 Université Paris-Saclay
5 University of Haifa [Haifa]
6 CMU - Carnegie Mellon University [Pittsburgh]
7 CAM - University of Cambridge [UK]
8 UC Davis - University of California [Davis]
9 UHH - Universität Hamburg
10 UTokyo - The University of Tokyo
11 UW - University of Warsaw
12 Karolinska Institutet [Stockholm]
13 Max Planck Research Group for RNA Biology
14 UIC - University of Illinois [Chicago]
15 HMS - Harvard Medical School [Boston]
16 Hubrecht Institute [Utrecht, Netherlands]
17 TU Delft - Delft University of Technology
18 McGill University = Université McGill [Montréal, Canada]
19 UNITN - Università degli Studi di Trento = University of Trento
20 JMU - Julius-Maximilians-Universität Würzburg = University of Würzburg
21 The University of Texas M.D. Anderson Cancer Center [Houston]
22 UND - University of Notre Dame [Indiana]
23 College of Life Sciences
24 University of Maryland [College Park]
25 University of Maryland [Baltimore]
26 Yale University [New Haven]
27 Brigham and Women's Hospital [Boston]
28 University of Colorado Anschutz [Aurora]
29 NYSBC - New York Structural Biology Center
30 UNIBE - Universität Bern / University of Bern
31 Texas A&M University [College Station]
32 Department of Biochemistry
33 Columbia University [New York]
34 University of Alberta
35 MBU / CAS - Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic]
Gary Loughran
  • Fonction : Auteur
  • PersonId : 1335821
Sergey E Dmitriev
  • Fonction : Auteur
  • PersonId : 1335822
Pavel V Baranov
  • Fonction : Auteur
  • PersonId : 1335823

Résumé

Dual reporters, which encode two distinct proteins within the same mRNA, have played a crucial role in identifying and characterizing new instances of unconventional eukaryotic translation mechanisms. These mechanisms include initiation by internal ribosomal entry sites (IRESs), ribosomal frameshifting, stop codon readthrough, and reinitiation. This design allows one reporter's expression to be influenced by the specific mechanism under investigation, while the other reporter serves as an internal control. However, challenges arise when intervening sequences are placed between these two reporters. Such sequences can inadvertently impact the expression or function of either reporter, independently of translation-related changes. These effects may occur because of cryptic elements inducing or affecting transcription initiation, splicing, polyadenylation, and antisense transcription, as well as unexpected effects of the translated test sequences on the stability and activity of the reporters. Unfortunately, these unintended effects may lead to incorrect conclusions being published in the scientific literature. To address this issue and assist the scientific community in accurately interpreting dual reporter experiments, we have developed comprehensive guidelines. These guidelines cover experiment design, interpretation, and the minimal requirements for reporting results. They are designed to aid researchers conducting these experiments, as well as reviewers, editors, and other investigators who seek to evaluate published data.
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Dates et versions

hal-04395528 , version 1 (15-01-2024)

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  • HAL Id : hal-04395528 , version 1

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Gary Loughran, Dmitry E Andreev, Ilya M Terenin, Olivier Namy, Martin Mikl, et al.. Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR). 2024. ⟨hal-04395528⟩

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