Regulatory T‐cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule‐1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Immunology Année : 2015

Regulatory T‐cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule‐1

Gaëlle Gottrand
  • Fonction : Auteur
Tristan Courau
  • Fonction : Auteur
Véronique Thomas-Vaslin
  • Fonction : Auteur
Nicolas Prevel
  • Fonction : Auteur
Thomas Vazquez
  • Fonction : Auteur
Maria Grazia Ruocco
  • Fonction : Auteur
Benedicte Lambrecht
  • Fonction : Auteur
Bertrand Bellier
  • Fonction : Auteur
David Klatzmann
  • Fonction : Auteur

Résumé

Summary To further investigate the contribution of intercellular adhesion molecule‐1 ( ICAM ‐1) to adaptive immune responses, we analysed T‐cell development and function in mice lacking full‐length ICAM ‐1 ( ICAM ‐1 tm1Jcgr ). Compared with wild‐type ( ICAM ‐1 WT ) mice, ICAM ‐1 tm1Jcgr mice have impaired thymocyte development. Proportions and numbers of double negative, double positive, mature CD 4 + and CD 8 + thymocytes, as well as of regulatory T (Treg) cells were also significantly decreased. In the periphery, ICAM ‐1 tm1Jcgr mice had significantly decreased proportions and numbers of naive and activated/memory CD 4 + and CD 8 + T cells, as well as of Treg cells, in lymph nodes but not in the spleen. In vitro activation of CD 4 + and CD 8 + T cells from ICAM ‐1 tm1Jcgr mice with anti‐ CD 3 antibodies and antigen‐presenting cells ( APC s) resulted in a significantly weaker proliferation, whereas proliferation induced with anti‐ CD 3 and anti‐ CD 28 antibody‐coated beads was normal. In vivo immunization of ICAM ‐1 tm1Jcgr mice resulted in normal generation of specific effector and memory immune responses that protect against a viral challenge. However, contrary to ICAM ‐1 WT mice, immunization‐induced specific effectors could not eradicate immunogen‐expressing tumours. Treg cells from ICAM ‐1 tm1Jcgr mice have abnormal activation and proliferation induced by anti‐ CD 3 antibody and APC s, and have markedly decreased suppressive activity in vitro . In contrast to ICAM ‐1 WT mice, they were unable to control experimentally induced colitis in vivo . Hence, our results further highlight the pleiotropic role of ICAM ‐1 in T‐cell‐dependent immune responses, with a major role in Treg cell development and suppressive function.

Dates et versions

hal-04393090 , version 1 (14-01-2024)

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Citer

Gaëlle Gottrand, Tristan Courau, Véronique Thomas-Vaslin, Nicolas Prevel, Thomas Vazquez, et al.. Regulatory T‐cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule‐1. Immunology, 2015, 146 (4), pp.657-670. ⟨10.1111/imm.12533⟩. ⟨hal-04393090⟩
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