Mammalian cell growth characterisation by a non-invasive plate reader assay - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2024

Mammalian cell growth characterisation by a non-invasive plate reader assay

Résumé

Automated and non-invasive mammalian cell analysis is currently lagging behind due to a lack of methods suitable for a variety of cell lines and applications. Here, we report the development of a high throughput non-invasive method for tracking mammalian cell growth and performance based on plate reader measurements. We show the method to be suitable for both suspension and adhesion cell lines, and we demonstrate it can be adopted when cells are grown under different environmental conditions. We establish that the method is suitable to inform on effective drug treatments to be used depending on the cell line considered, and that it can support characterisation of engineered mammalian cells over time. This work provides the scientific community with an innovative approach to mammalian cell screening, also contributing to the current efforts towards high throughput and automated mammalian cell engineering.
Fichier principal
Vignette du fichier
s41467-023-44396-4.pdf (1008.95 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-04392382 , version 1 (08-02-2024)

Licence

Identifiants

Citer

Alice Grob, Chiara Enrico Bena, Roberto Di Blasi, Daniele Pessina, Matthew Sood, et al.. Mammalian cell growth characterisation by a non-invasive plate reader assay. Nature Communications, 2024, 15 (1), pp. 57. ⟨10.1038/s41467-023-44396-4⟩. ⟨hal-04392382⟩
39 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More