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Article Dans Une Revue Cells Année : 2021

Functional Ex Vivo Testing of Alveolar Monocytes in Patients with Pneumonia-Related ARDS

Inès Bendib
  • Fonction : Auteur
Asma Beldi-Ferchiou
  • Fonction : Auteur
Frédéric Schlemmer
  • Fonction : Auteur
Bernard Maitre
  • Fonction : Auteur
Mathieu Surenaud
  • Fonction : Auteur
Sophie Hüe
  • Fonction : Auteur
Guillaume Carteaux
  • Fonction : Auteur
Keyvan Razazi
  • Fonction : Auteur
Jean-Daniel Lelièvre
Yves Lévy
  • Fonction : Auteur
Véronique Godot
Nicolas de Prost

Résumé

Biomarkers of disease severity might help with individualizing the management of patients with acute respiratory distress syndrome (ARDS). During sepsis, a sustained decreased expression of the antigen-presenting molecule human leucocyte antigen-DR (HLA-DR) on circulating monocytes is used as a surrogate marker of immune failure. This study aimed at assessing whether HLA-DR expression on alveolar monocytes in the setting of a severe lung infection is associated with their functional alterations. BAL fluid and blood from immunocompetent patients with pneumonia-related ARDS admitted between 2016 and 2018 were isolated in a prospective monocentric study. Alveolar and blood monocytes were immunophenotyped using flow cytometry. Functional tests were performed on alveolar and blood monocytes after in vitro lipopolysaccharide (LPS) stimulation. Phagocytosis activity and intracellular tumor necrosis factor (TNF) production were quantified using fluorochrome-conjugated-specific antibodies. Ten ARDS and seven non-ARDS control patients were included. Patients with pneumonia-related ARDS exhibited significantly lower HLA-DR expression both on circulating (p < 0.0001) and alveolar (p = 0.0002) monocytes. There was no statistically significant difference observed between patient groups (ARDS vs. non-ARDS) regarding both alveolar and blood monocytes phagocytosis activity. After LPS stimulation, alveolar (p = 0.027) and blood (p = 0.005) monocytes from pneumonia-related ARDS patients had a significantly lower intracellular TNF expression than non-ARDS patients. Monocytes from pneumonia-related ARDS patients have a deactivated status and an impaired TNF production capacity but display potent phagocytic activity. HLA-DR level expression should not be used as a surrogate marker of the phagocytic activity or the TNF production capacity of alveolar monocytes.

Dates et versions

hal-04392278 , version 1 (13-01-2024)

Identifiants

Citer

Inès Bendib, Asma Beldi-Ferchiou, Frédéric Schlemmer, Bernard Maitre, Mathieu Surenaud, et al.. Functional Ex Vivo Testing of Alveolar Monocytes in Patients with Pneumonia-Related ARDS. Cells, 2021, 10 (12), pp.3546. ⟨10.3390/cells10123546⟩. ⟨hal-04392278⟩

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