FROGS
Résumé
FROGS is a CLI workflow designed to produce an ASV count matrix from high depth sequencing amplicon data.
FROGS-wrappers allow to add FROGS on a Galaxy instance. (see https://github.com/geraldinepascal/FROGS-wrappers)
This workflow is focused on:
- User-friendliness with lots of rich graphic outputs and the integration in Galaxy thanks to FROGS-wrappers.
- Accuracy with a clustering without global similarity threshold, the management of separated PCRs in the chimera removal step, and the management of multi-affiliations.
- Dealing of non overlapping pair of sequences from long amplicon like ITS, or RPB2.
- Access to general statistics on microbial communities and differential abundance analysis.
- Prediction of functionnal annotation and metabolic pathways.
- Speed with fast algorithms parallelisation and easy to use.
- Scalability with algorithms designed to support the data growth.
