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Logiciel Année : 2024

FROGS

Résumé

FROGS is a CLI workflow designed to produce an ASV count matrix from high depth sequencing amplicon data. FROGS-wrappers allow to add FROGS on a Galaxy instance. (see https://github.com/geraldinepascal/FROGS-wrappers) This workflow is focused on: - User-friendliness with lots of rich graphic outputs and the integration in Galaxy thanks to FROGS-wrappers. - Accuracy with a clustering without global similarity threshold, the management of separated PCRs in the chimera removal step, and the management of multi-affiliations. - Dealing of non overlapping pair of sequences from long amplicon like ITS, or RPB2. - Access to general statistics on microbial communities and differential abundance analysis. - Prediction of functionnal annotation and metabolic pathways. - Speed with fast algorithms parallelisation and easy to use. - Scalability with algorithms designed to support the data growth.
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