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Poster De Conférence Année : 2023

Strategy using a combination of physico/chemical analysis and in vitro bioassays for chemical safety of food contact articles

Résumé

Conducting risk assessments for Non-Intentionally Added Substances (NIAS) in food contact articles (FCA) that may migrate into food presents a significant challenge for food packaging manufacturers, as well as for risk assessors and managers. This challenge becomes even more pronounced in the context of a circular economy where the utilization of bio-based, reusable, and recycled materials is expected to surge in the coming years. The objective of the Packsafe project is to propose a comprehensive strategy for ensuring the chemical safety of FCAs. This strategy involves the combination of analytical tools and in vitro studies, enabling the identification of hazards within complex mixtures. The project involves extracting various FCAs and subjecting them to thorough analysis through analytical chemistry to establish a unique chemical signature or fingerprint. Simultaneously, these extracts will undergo in vitro biotests to assess genotoxicity (Ames test, micronucleus assay, DDR test) and endocrine disruption (employing an Estrogen/Androgen/Thyroid/Steroidogenesis approach). The initial phase of the project entailed extracting forty-one articles, including LDPE, HDPE, PP, PET, PS, and cardboard. The extraction protocol involved a mixture of methanol and dichloromethane (1/3), followed by a concentration step to transfer the substances into a biocompatible solvent (DMSO). These mixtures were then subjected to a battery of in vitro bioassays. Positive results were observed in some cases, suggesting potential genotoxicity and endocrine disruption, which will require further confirmation. For extracts with negative results, additional spiked experiments will be conducted to study the matrix effect of the extract and determine the threshold of biological detection. The subsequent phase of the project involves the preparation of extracts enriched with NIAS, which will be tested using selected biotests. Concurrently, chemical analysis will be carried out to characterize the extracts and attempt to identify their chemical signature. All the collected data will be integrated into an expert system, which will serve as a comprehensive toolbox for risk assessment.
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Dates et versions

hal-04375350 , version 1 (05-01-2024)

Identifiants

  • HAL Id : hal-04375350 , version 1

Citer

Isabelle Séverin, Laurence Dahbi, Sandra Domenek, Phuong-Mai Nguyen, Anne Platel, et al.. Strategy using a combination of physico/chemical analysis and in vitro bioassays for chemical safety of food contact articles. RiskTOX 2023, Nov 2023, Valencia (Espagne), Spain. ⟨hal-04375350⟩
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