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Pré-Publication, Document De Travail Année : 2023

A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana

Sébastien Carrère
Jean-Marc Routaboul
  • Fonction : Auteur
Pauline Savourat
  • Fonction : Auteur
Caroline Bellenot
  • Fonction : Auteur
Hernán López
  • Fonction : Auteur
Thomas Quiroz Monnens
  • Fonction : Auteur
Anthony Ricou
  • Fonction : Auteur
Christine Camilleri
  • Fonction : Auteur
Patrick Laufs
Raphael Mercier
Laurent Noël
  • Fonction : Auteur

Résumé

Abstract Genetic screens are powerful tools for biological research and are one of the reasons for the success of the thale cress Arabidopsis thaliana as a model species. Here, we describe the whole-genome sequencing of 871 Arabidopsis lines from the Homozygous EMS Mutant (HEM) collection as a novel resource for forward and reverse genetics. With an average 576 high-confidence mutations per HEM line, over three independent mutations altering protein sequence are found on average per gene in the collection. Pilot reverse genetics experiments on reproductive, developmental and physiological traits confirmed the efficacy of the tool for identifying both null and knockdown alleles. The possibility of conducting subtle repeated phenotyping of HEM lines and the immediate availability of the mutations will empower forward genetic approaches. The sequence resource is searchable with the ATHEM web interface ( https://lipm-browsers.toulouse.inra.fr/pub/ATHEM/ ), and the biological material is distributed by the Versailles Arabidopsis Stock Center.

Dates et versions

hal-04372773 , version 1 (04-01-2024)

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Sébastien Carrère, Jean-Marc Routaboul, Pauline Savourat, Caroline Bellenot, Hernán López, et al.. A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana. 2023. ⟨hal-04372773⟩
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