P17-35 Characterization of in-situ derived metallic nanoparticles from implant wear as a prerequisite for toxicity assessment - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

P17-35 Characterization of in-situ derived metallic nanoparticles from implant wear as a prerequisite for toxicity assessment

Résumé

Background: Metallic micro- and nanoparticles can be generated on the articulating surfaces and modular junctions of orthopedic implants [1]. The in situ formation of wear particles represents a unique toxicological exposure scenario and is associated with adverse local tissue reactions (ALTR) that can lead to osteolysis and aseptic implant loosening while the underlying mechanisms are not fully understood [2]. The toxicity of micro- and nanoparticles is known to be a function of their shape, size, Methods: Sample collection was performed during implant revision surgeries that were conducted due to signs of ALTR. Acquisition of samples focused on implants that were withdrawn from the market due to poor performance. Micro- and Nanoparticles were purified using a two-step enzymatic digestion protocol. The samples were pooled according to the implant type they are derived from with n=11 samples for Metal-on-Metal (MoM) total hip arthroplasty (THA) with CoCrMo metallic adapter sleeve (MAS), n=11 Results: Particles were isolated from each group in different quantities. The wear particles in each group showed a large heterogeneity in size and morphology, ranging from round or elliptic to needle/rectangular shapes. The range of particle sizes and their elemental composition was different for each implant type. In addition to single dispersed particles, agglomerates/aggregates of micro- and nanoparticles were found in all samples. Introduction to biological fluids was accompanied by an increase in Conclusion: The presented results provide a solid basis for further research focusing on micro- and nanoparticle toxicity. In addition, the need for particle characterization in biological fluids that reflect their environment in situ is emphasized, since physicochemical properties strongly depended on the respective liquid. Further investigation of micro- and nanoparticle toxicity in vitro will help to correlate their physicochemical properties to observed pathologies such as ALTRs.
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Dates et versions

hal-04371008 , version 1 (03-01-2024)

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F. Schulze, Lionel Maurizi, A. Rakow, J. Schoon, G. Wassilew, et al.. P17-35 Characterization of in-situ derived metallic nanoparticles from implant wear as a prerequisite for toxicity assessment. 57th Congress of the European Societies of Toxicology (EUROTOX 2023): TOXICOLOGY - MULTIDISCIPLINARY SCIENCE LEADING TO SAFER AND SUSTAINABLE LIFE, Sep 2023, Ljubljana, Slovenia. pp.S221-S222, ⟨10.1016/S0378-4274(23)00776-2⟩. ⟨hal-04371008⟩
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