B- and T-cell lymphocytes and other immune cell infiltration in the duodenal and rectal mucosa of severe asthmatic horses - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue American Journal of Veterinary Research Année : 2023

B- and T-cell lymphocytes and other immune cell infiltration in the duodenal and rectal mucosa of severe asthmatic horses

Céline Loubière
  • Fonction : Auteur
Rullier Marine
  • Fonction : Auteur
Pierre Hélie
  • Fonction : Auteur
Daniel Jean
  • Fonction : Auteur

Résumé

Abstract OBJECTIVE The objectives of this study were to quantify lymphocytes and eosinophils in the mucosa of the duodenum and rectum in asthmatic horses. ANIMALS 8 healthy and 10 asthmatic horses. PROCEDURES Asthmatic horses were evaluated in a symptomatic (after 6 weeks of exposure to moldy hay) and asymptomatic status (3 and 7 months after being fed alfalfa pellets [n = 4] or treated with inhaled fluticasone [6]). Duodenal and rectal biopsies were endoscopically (n = 4 to 6) taken in each horse. Eosinophils were counted on slides stained with hematoxylin, eosin, phloxine, and saffron, and immunohistochemistry was used to evaluate T and B lymphocytes using CD3 and CD20, respectively. RESULTS The duodenal and rectal epithelium of asthmatic and control horses contained exclusively T lymphocytes (CD3). Symptomatic asthmatic horses, compared to controls, had a significantly higher number of T lymphocytes (CD3) in the duodenal epithelium ( P = .016) and the adjacent lamina propria of the villi ( P = .04). Compared to symptomatic asthmatic horses, the fluticasone-treated group had significantly fewer T lymphocytes in the total lamina propria of the rectal mucosa ( P < .01). CLINICAL RELEVANCE Taken together, these results suggest that asthmatic horses have greater infiltration of T lymphocytes in the duodenal and rectal mucosa, indicating a certain degree of inflammation, which could be due to a systemic inflammatory effect and/or a local effect of ingested hay allergens in asthmatic horses. Systemic markers of inflammation have not been investigated to better qualify if the infiltration noted is due to a local and/or systemic effect.

Dates et versions

hal-04369078 , version 1 (02-01-2024)

Identifiants

Citer

Céline Loubière, Peggy Moreau, Rullier Marine, Pierre Hélie, Daniel Jean. B- and T-cell lymphocytes and other immune cell infiltration in the duodenal and rectal mucosa of severe asthmatic horses. American Journal of Veterinary Research, 2023, 84 (1), pp.1-10. ⟨10.2460/ajvr.23.04.0083⟩. ⟨hal-04369078⟩

Collections

ANSES
10 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More