The charring behaviour of timber elements under fire is well understood, however, the effects of fire and heat on connections are not equally well known. Timber connections often use steel fasteners, like screws or angle brackets, which conduct heat much better than wood. Moreover, these fasteners lose their mechanical resistance and capacity under elevated temperatures. X-ray computed tomography (CT) can be used to reconstruct the internal structure of wood non-destructively. It should therefore be possible to use this technology to also study the progressive degradation due to fire of a timber connection. The goal of the present study is to investigate how CT can be used to analyse the degradation of a timber connection due to fire. Samples of Norway spruce with self-tapping screws were scanned before and after a fire exposure, and mechanical tests were performed. The results indicate that the degradation due to fire in a timber connection can be observed in CT scans, but that certain measures need to be taken to minimise the effects of image artefacts due to X-ray scattering and photon starvation.