Effect of micronutrients and L-carnitine as antioxidant on sperm parameters, genome integrity, and ICSI outcomes: randomized, double-blind, and placebo-controlled clinical trial - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Antioxidants Année : 2023

Effect of micronutrients and L-carnitine as antioxidant on sperm parameters, genome integrity, and ICSI outcomes: randomized, double-blind, and placebo-controlled clinical trial

Résumé

The evaluation of sperm DNA integrity is recommended in the sixth edition of the 2021 World Health Organization guidelines. Oxidative stress has been identified as a crucial factor leading to genome decay, lipid peroxidation, and nucleoprotein oxidation. This double-blind, placebo-controlled clinical trial aimed to assess the effect of oral antioxidant treatment (Fertilis), which contains L-carnitine and some micronutrients, in the improvement of conventional sperm parameters, sperm DNA integrity and in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes. A total of 263 participants were enrolled and randomly divided into two groups: 131 participants received the antioxidant treatment, while 132 participants received a placebo. The male partners in both groups underwent the antioxidant treatment or the placebo for a duration of three months. For each participant, we performed a hormonal test, an infectious test, a spermogram, a TUNEL assay for sperm DNA fragmentation, a toluidine blue staining for sperm DNA decondensation, and an IVF/ICSI procedure. Sperm characteristics analysis (volume, count, motility, and vitality), sperm DNA fragmentation, and sperm DNA decondensation were assessed and compared to the results preceding the antioxidant treatment. The study outcome revealed a significant decrease in the DNA fragmentation index and a significant increase in sperm motility after 3 months of treatment (p = 0.01 and p = 0.02, respectively). Additionally, a significant improvement in clinical pregnancy rate (p = 0.01) and life birth rate (p = 0.031) was observed. No significant changes were observed in conventional sperm parameters (volume, count, and vitality) or sperm DNA decondensation (SDI). Antioxidant therapy has a beneficial impact on achieving pregnancy, whether through spontaneous conception or assisted reproductive procedures (ART).

Dates et versions

hal-04358122 , version 1 (21-12-2023)

Identifiants

Citer

Marwa Lahimer, Oumaima Gherissi, Nesrine Ben Salem, Henda Ben Mustapha, Véronique Bach, et al.. Effect of micronutrients and L-carnitine as antioxidant on sperm parameters, genome integrity, and ICSI outcomes: randomized, double-blind, and placebo-controlled clinical trial. Antioxidants , 2023, 12 (11), pp.1937. ⟨10.3390/antiox12111937⟩. ⟨hal-04358122⟩

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