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Article Dans Une Revue Biosensors and Bioelectronics Année : 2004

A bioelectrochemical polypyrrole-containing Fe(CN)63- interface for the design of a NAD-dependent reagentless biosensor

Pierre Gros

Résumé

Ferricyanide ions were immobilized on a platinum electrode surface by means of an electrochemically grown polypyrrole film. The entrapped Fe(CN)63−/Fe(CN)64− redox system displayed a high heterogeneous electron transfer rate. The resulting modified electrode was efficient for the ferricyanide-mediated NADH oxidation catalyzed by a diaphorase. The bioelectrochemical interface was applied to the design of a reagentless amperometric d-lactate biosensor. A weakly polarized two polypyrrole-containing Fe(CN)63− modified electrode system was involved without any reference. An enzymatic solution containing d-lactate dehydrogenase, diaphorase and NAD-dextran was further confined on the sensing electrode using a semi-permeable membrane. The sensitivity and the response time of the reagentless biosensor were similar to those of the analogous sensor working with soluble mediator and cofactor, i.e. 25 μA mM−1 cm−2 and 120 s, respectively. The other analytical performances were less satisfactorily: the detection limit was 5×10−2 mmol L−1 and the linearity range was comprised between 0.1 and 0.5 mmol L−1.

Dates et versions

hal-04347534 , version 1 (15-12-2023)

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Pierre Gros, Maurice Comtat. A bioelectrochemical polypyrrole-containing Fe(CN)63- interface for the design of a NAD-dependent reagentless biosensor. Biosensors and Bioelectronics, 2004, 20 (2), pp.204-210. ⟨10.1016/j.bios.2004.02.023⟩. ⟨hal-04347534⟩
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