An amperometric silicon-based biosensor for d-lactate - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Sensors and Actuators B: Chemical Année : 2000

An amperometric silicon-based biosensor for d-lactate

Résumé

A miniaturised biosensor for d-lactate has been fabricated on silicon wafer. Microsystems technologies led to enhanced efficiency in terms of sample size and reagent consumption while equally allowing mass production. The biosensor is based on the association of an enzymatic solution including diaphorase and d-lactate dehydrogenase confined in the microstructure by a membrane and two weakly polarised gold microelectrodes. Various electrochemical pretreatments of the gold surface are performed to increase the device's sensitivity. Depending on the geometry involved, the proposed biosensor features a response time ranging from 2 to 5 min, a sensitivity of 2 or 8 μA mM−1 cm−2 and a linearity range from 5×10−3 to 1.5 mmol l−1. Its lifetime is 3 weeks with 10 assays/day.

Dates et versions

hal-04347462 , version 1 (15-12-2023)

Identifiants

Citer

Hélène Tap, Pierre Gros, A.M Gué. An amperometric silicon-based biosensor for d-lactate. Sensors and Actuators B: Chemical, 2000, 68 (1-3), pp.123-127. ⟨10.1016/S0925-4005(00)00471-8⟩. ⟨hal-04347462⟩
9 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More