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Article Dans Une Revue Electroanalysis Année : 1999

Design of a Silicon Based Amperometric Microbiosensor Involving NAD-Dependent Dehydrogenase

Hélène Tap
Pierre Gros

Résumé

An electrochemical microbiosensor involving NAD-dependent dehydrogenase has been designed and developed using silicon technology. The sensor is based on a weakly polarized two-gold microelectrode system without any reference electrode. The process allowed mass production of miniaturized devices. An enzymatic solution including diaphorase and NAD-dependent dehydrogenase was confined in the microstructure by means of a semi-permeable membrane. The biocompatibility of the various materials was verified and the gold electrodes were electrochemically characterized. The assay of D-lactic acid was taken as an example using D-lactate dehydrogenase. The sensitivity and detection limit of the microbiosensor were 8 μA mM–1 cm–2 and 0.01 mM respectively. Its lifetime was 3 weeks.

Dates et versions

hal-04347426 , version 1 (15-12-2023)

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Citer

Hélène Tap, Pierre Gros, Anne-Marie Gué. Design of a Silicon Based Amperometric Microbiosensor Involving NAD-Dependent Dehydrogenase. Electroanalysis, 1999, 11 (13), pp.973-977. ⟨10.1002/(SICI)1521-4109(199909)11:13<973::AID-ELAN973>3.0.CO;2-3⟩. ⟨hal-04347426⟩
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