Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Fungal Genetics and Biology Année : 2009

Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices

Résumé

Sterols are crucial components of eukaryotic membranes that control membrane fluidity and permeability. They play an important role in cell signaling, polarity and sorting. Since many steps in the pathway are essential, sterol biosynthesis inhibitors (SBI) are widely used as antifungal agents. This work reports the identification and the characterization of a C-4 sterol methyl oxidase (SMO), the first gene involved in the sterol biosynthetic pathway, so far described from an arbuscular mycorrhizal fungus. The sequence, called GintSMO, shows a primary structure, a hydrophobicity profile and a pattern of histidine-rich motifs which are typical of C-4 methyl sterol oxidases. The complementation assay in a Saccharomyces cerevisiae mutant strain demonstrates that GintSMO encodes a functional SMO. Changes in GintSMO transcript levels and in the amount of the sterol precursor squalene were observed in in vitro grown extraradical structures exposed to the fenpropimorph SBI fungicide.

Dates et versions

hal-04322628 , version 1 (05-12-2023)

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Elodie Oger, Stefano Ghignone, Estelle Campagnac, Luisa Lanfranco, Joël Fontaine, et al.. Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices. Fungal Genetics and Biology, 2009, 46 (6-7), pp.486-495. ⟨10.1016/j.fgb.2009.03.002⟩. ⟨hal-04322628⟩
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