Understanding the spatio-temporal distribution of species is a cornerstone of ecology and conservation. By pairing species observations with geographic and environmental predictors, researchers can model the relationship between an environment and the species which may be found there. To advance the stateof-the-art in this area with deep learning models and remote sensing data, we organized an open machine learning challenge called GeoLifeCLEF 2023. The training dataset comprised 5 million plant species observations (single positive label per sample) distributed across Europe and covering most of its flora, high-resolution rasters: remote sensing imagery, land cover, elevation, in addition to coarse-resolution data: climate, soil and human footprint variables. In this multi-label classification task, we evaluated models ability to predict the species composition in 22 thousand small plots based on standardized surveys. This paper presents an overview of the competition, synthesizes the approaches used by the participating teams, and analyzes the main results. In particular, we highlight the biases faced by the methods fitted to single positive labels when it comes to the multi-label evaluation, and the new and effective learning strategy combining single and multi-label data in training.