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Poster De Conférence Année : 2023

Greedy reduction of Bacillus subtilis genome yields emergent phenotypes

Kosei Tanaka
  • Fonction : Auteur
  • PersonId : 969464
Victor Chubukov
  • Fonction : Auteur
Uwe Sauer
  • Fonction : Auteur
  • PersonId : 841574
Ken-Ichi Yoshida
  • Fonction : Auteur

Résumé

We investigate the consequences of genome reduction in Bacillus subtilis, which has many advantages for genome engineering including natural competence, efficient chromosomal replacement, existing repertoires of non-essential regions and genes, and availability of libraries of parts for fine-tuning gene circuit expression. As such, B. subtilis is an ideal candidate for the development of a well-characterized minimal chassis in which complex metabolic and regulatory circuits can be implemented and fine-tuned. First, we applied a greedy genome reduction strategy in which deletions in large dispensable regions were serially combined starting from the largest. At each step, the fittest clonal population was used for the next deletion cycle. This strategy generated a synthetic evolutionary tree of 298 strains with reduced genomes, the smallest genome (of 2.73 Mb) having lost 1.48 Mb in size.

Domaines

Autre [q-bio.OT]
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Dates et versions

hal-04316425 , version 1 (30-11-2023)

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  • HAL Id : hal-04316425 , version 1

Citer

Etienne Dervyn, Anne-Gaëlle Planson, Kosei Tanaka, Victor Chubukov, Cyprien Guérin, et al.. Greedy reduction of Bacillus subtilis genome yields emergent phenotypes. BACELL2023, Nov 2023, Kobe, Japan. . ⟨hal-04316425⟩
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