IN DICKEYA DADANTI, THE TYPE II SECRETION SYSTEM (T2SS) IS COVALENTLY ATTACHED TO THE BACTERIAL CELL WALL - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2023

IN DICKEYA DADANTI, THE TYPE II SECRETION SYSTEM (T2SS) IS COVALENTLY ATTACHED TO THE BACTERIAL CELL WALL

Résumé

Dickeya dadantii is a broad-spectrum phytopathogenic γ-proteobacterium that provokes soft rot disease in various plants. D. dadantii pathogenicity is caused by an arsenal of plant cellwall degrading enzymes secreted by the Type II Secretion System (T2SS) named Out. The Out system is a multiprotein complex spanning the cell envelope. Recently, we showed that OutB, an inner membrane T2SS component plays an important scaffolding role and that deletion of outB causes a striking reduction of pectinase secretion and virulence of D. dadantii. Here, we show that in D. dadantii, OutB is covalently attached to the PG. We investigated the molecular mechanisms of OutB attachment to peptidoglycan (PG) and the possible implication of this phenomenon in the virulence of D. dadantii. First, the covalent attachment of OutB was demonstrated after extraction, purification and analysis of the PG of D. dadantii by Western blot. Second, we characterized the enzymes implicated and identified the region and residues of OutB important for covalent attachment to PG: the two C-terminal lysines of OutB are involved in its attachment to PG and two L,D-transpeptidases, Ldt03 and Ldt84, catalyze this process. These results suggest that the covalent attachment of OutB to PG allows a better stabilization of the T2SS in the bacterial cell envelope. Moreover, it shows that the phenomenon of covalent attachment of proteins to PG in proteobacteria could be more widespread and remains largely unexplored.

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Dates et versions

hal-04309299 , version 1 (27-11-2023)

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  • HAL Id : hal-04309299 , version 1

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Xavier Nicolai, Vladimir E Shevchik. IN DICKEYA DADANTI, THE TYPE II SECRETION SYSTEM (T2SS) IS COVALENTLY ATTACHED TO THE BACTERIAL CELL WALL. 12th INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, Aug 2023, Lyon, France. 2023. ⟨hal-04309299⟩
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