Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Communications Biology Année : 2023

Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model

Résumé

Lysosomes orchestrate degradation and recycling of exogenous and endogenous material thus controlling cellular homeostasis. Little is known how this organelle changes during cancer. Here we investigate the intracellular landscape of lysosomes in a cellular model of bladder cancer. Employing standardized cell culture on micropatterns we identify a phenotype of peripheral lysosome positioning prevailing in bladder cancer cell lines but not normal urothelium. We show that lysosome positioning is controlled by phosphatidylinositol-3-phosphate (PtdIns3P) levels on endomembranes which recruit FYVE-domain containing proteins for lysosomal dispersion. We identify transcription factor EB (TFEB) as an upstream regulator of PtdIns3P production by VPS34 that is activated in aggressive bladder cancer cells with peripheral lysosomes. This conceptually clarifies the dual role of TFEB as regulator of endosomal maturation and autophagy, two distinct processes controlled by PtdIns3P. Altogether, our findings uncover peripheral lysosome positioning, resulting from PtdIns3P production downstream of TFEB activation, as a potential biomarker for bladder cancer.
Fichier principal
Vignette du fichier
s42003-023-04501-1.pdf (6.91 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-04308552 , version 1 (27-11-2023)

Identifiants

Citer

Pallavi Mathur, Camilla de Barros Santos, Hugo Lachuer, Julie Patat, Bruno Latgé, et al.. Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model. Communications Biology, 2023, 6, pp.114. ⟨10.1038/s42003-023-04501-1⟩. ⟨hal-04308552⟩
15 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More