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Article Dans Une Revue Biotechnology and Bioengineering Année : 2023

Acoustic levitation as a tool for cell-driven self-organization of human cell spheroids during long-term 3D culture

Résumé

Acoustic levitation, which allows contactless manipulation of micro-objects with ultrasounds, is a promising technique for spheroids formation and culture. This acoustofluidic technique favors cellcell interactions, away from the walls of the chip, which leads to the spontaneous self-organization of cells. Using this approach, we generated spheroids of mesenchymal stromal cells, hepatic and endothelial cells, and showed that long-term culture of cells in acoustic levitation is feasible. We also demonstrated that this self-organization and its dynamics depended weakly on the acoustic parameters but was strongly dependent on the levitated cell type. Moreover, spheroid organization was modified by actin cytoskeleton inhibitors or calcium-mediated interactions inhibitors. Our results confirmed that acoustic levitation is a rising technique for fundamental research and biotechnological industrial application in the rapidly growing field of microphysiological systems. It allowed easily obtaining spheroids of specific and predictable shape and size, which could be cultivated over several days, without requiring hydrogels or extracellular matrix.
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hal-04307979 , version 1 (26-11-2023)

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  • HAL Id : hal-04307979 , version 1

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Lucile Rabiet, Lousineh Arakelian, Nathan Jeger-Madiot, Duvan Rojas Garcia, Jerome Larghero, et al.. Acoustic levitation as a tool for cell-driven self-organization of human cell spheroids during long-term 3D culture. Biotechnology and Bioengineering, In press. ⟨hal-04307979⟩
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