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Article Dans Une Revue Microbiology Année : 2006

Identification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA gene

Gerard Mazón
Susana Campoy
  • Fonction : Auteur
Ivan Erill
  • Fonction : Auteur
Jordi Barbé
  • Fonction : Auteur

Résumé

Acidobacterium capsulatum is the most thoroughly studied species of a new bacterial phylogenetic group designated the phylum Acidobacteria. Through a tblastn search, the A. capsulatum lexA gene has been identified, and its product purified. Electrophoretic mobility shift assays have shown that A. capsulatum LexA protein binds specifically to the direct repeat GTTCN 7 GTTC motif. Strikingly, this is also the LexA box of the Alphaproteobacteria, but had not previously been described outside this subclass of the Proteobacteria. In addition, a phylogenetic analysis of the LexA protein clusters together Acidobacterium and the Alphaproteobacteria, moving the latter away from their established phylogenetic position as a subclass of the Proteobacteria, and pointing to a lateral gene transfer of the lexA gene from the phylum Acidobacteria, or an immediate ancestor, to the Alphaproteobacteria. Lastly, in vivo experiments demonstrate that the A. capsulatum recA gene is DNA-damage inducible, despite the fact that a LexA-binding sequence is not present in its promoter region.

Dates et versions

hal-04306400 , version 1 (24-11-2023)

Identifiants

Citer

Gerard Mazón, Susana Campoy, Ivan Erill, Jordi Barbé. Identification of the Acidobacterium capsulatum LexA box reveals a lateral acquisition of the Alphaproteobacteria lexA gene. Microbiology, 2006, 152 (4), pp.1109-1118. ⟨10.1099/mic.0.28376-0⟩. ⟨hal-04306400⟩
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