S‐Nitrosylation of the histone deacetylase HDA19 stimulates its activity to enhance plant stress tolerance in Arabidopsis
Résumé
Arabidopsis histone deacetylase HDA19 is required for gene expression programs of a large
spectrum of plant developmental and stress-responsive pathways. How this enzyme senses
cellular environment to control its activity remains unclear. In this work, we show that HDA19
is post-translationally modified by S-nitrosylation at 4 Cysteine (Cys) residues. HDA19 S-
nitrosylation depends on the cellular nitric oxide (NO) level which is enhanced under oxidative
stress. We find that HDA19 is required for cellular redox homeostasis and plant tolerance to
oxidative stress which in turn stimulates its nuclear enrichment, S-nitrosylation and epigenetic
functions including binding to genomic targets, histone deacetylation, and gene repression. The
Cys137 of the protein is involved in basal and stress-induced S-nitrosylation and is required for
HDA19 functions in developmental, stress-responsive, and epigenetic controls. Together, these
results indicate that S-nitrosylation regulates HDA19 activity and is a mechanism of redox-
sensing for chromatin regulation of plant tolerance to stress.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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