High and low permeability of human pluripotent stem cell–derived blood–brain barrier models depend on epithelial or endothelial features
Résumé
Abstract The search for reliable human blood–brain barrier (BBB) models represents a challenge for the development/testing of strategies aiming to enhance brain delivery of drugs. Human‐induced pluripotent stem cells (hiPSCs) have raised hopes in the development of predictive BBB models. Differentiating strategies are thus required to generate endothelial cells (ECs), a major component of the BBB. Several hiPSC‐based protocols have reported the generation of in vitro models with significant differences in barrier properties. We studied in depth the properties of iPSCs byproducts from two protocols that have been established to yield these in vitro barrier models. Our analysis/study reveals that iPSCs derivatives endowed with EC features yield high permeability models while the cells that exhibit outstanding barrier properties show principally epithelial cell‐like (EpC) features. We found that models containing EpC‐like cells express tight junction proteins, transporters/efflux pumps and display a high functional tightness with very low permeability, which are features commonly shared between BBB and epithelial barriers. Our study demonstrates that hiPSC‐based BBB models need extensive characterization beforehand and that a reliable human BBB model containing EC‐like cells and displaying low permeability is still needed.
Mots clés
BSA, bovine serum albumin
CNS, central nervous system
CspA, cyclosporin A
EC, endothelial cell
ECEM, endothelial cell expansion medium
ECIM, endothelial cell induction medium
ECM, extracelullar matrix
EpC, epithelial cell
FPKM, fragment per kilobase million
GSK3β, glycogen synthase kinase 3 beta
HBMVECs, human brain microvascular endothelial cells
hiPSCs, human-induced pluripotent stem cells
HUVECs, human umbilical vein endothelial cells
LY, lucifer yellow
MACS, magnetic activated cell sorting
NVU, neurovascular unit
Pe, permeability
Pr-1, protocol 1
PSCs, pluripotent stem cells
RA, retinoic acid
RPKM, read per kilobase million
TEER, trans-endothelial electrical resistance
TGFβ, transforming growth factor beta
TNF-α, tumor necrosis factor-alpha
Ac-LDL, acetylated low-density lipoprotein BBB, blood-brain barrier bFGF, basic fibroblast growth factor BMP4, bone morphogenic protein 4 BMVEC, brain microvascular endothelial cells BSA, bovine serum albumin CNS, central nervous system CspA, cyclosporin A EC, endothelial cell ECEM, endothelial cell expansion medium ECIM, endothelial cell induction medium ECM, extracelullar matrix EpC, epithelial cell FPKM, fragment per kilobase million GSK3β, glycogen synthase kinase 3 beta HBMVECs, human brain microvascular endothelial cells hiPSCs, human-induced pluripotent stem cells HUVECs, human umbilical vein endothelial cells LY, lucifer yellow MACS, magnetic activated cell sorting NVU, neurovascular unit Pe, permeability Pr-1, protocol 1 PSCs, pluripotent stem cells RA, retinoic acid RPKM, read per kilobase million TEER, trans-endothelial electrical resistance TGFβ, transforming growth factor beta TNF-α, tumor necrosis factor-alpha UM, unconditioned medium VEGF, vascular endothelial growth factor
UM, unconditioned medium
Ac-LDL, acetylated low-density lipoprotein
VEGF, vascular endothelial growth factor
BBB, blood-brain barrier
bFGF, basic fibroblast growth factor
BMP4, bone morphogenic protein 4
BMVEC, brain microvascular endothelial cells
BSA, bovine serum albumin
BSA, bovine serum albumin
BSA, bovine serum albumin
Domaines
Sciences du Vivant [q-bio]
Fichier principal
The FASEB Journal - 2023 - Girard - High and low permeability of human pluripotent stem cell derived blood brain barrier.pdf (11.27 Mo)
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