Super-resolution capacity of variance-based stochastic fluorescence microscopy
Résumé
Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achieved
by acquiring and processing multiple images of the sample under different illumination conditions.
One of the simplest techniques, Random Illumination Microscopy (RIM), forms the super-resolved
image from the variance of images obtained with random speckled illuminations. However, the
validity of this process has not been fully theorized. In this work, we characterize mathematically
the sample information contained in the variance of diffraction-limited speckled images as a function
of the statistical properties of the illuminations. We show that an unambiguous two-fold resolution
gain is obtained when the speckle correlation length coincides with the width of the observation
point spread function. Last, we analyze the difference between the variance-based techniques using
random speckled illuminations (as in RIM) and those obtained using random fluorophore activation
(as in Super-resolution Optical Fluctuation Imaging, SOFI).
Origine | Fichiers produits par l'(les) auteur(s) |
---|