Molecular Rearrangements in Protomembrane Models Probed by Laurdan Fluorescence - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Membranes Année : 2023

Molecular Rearrangements in Protomembrane Models Probed by Laurdan Fluorescence

Résumé

Lipid membranes are a key component of living systems and have been essential to the origin of life. One hypothesis for the origin of life assumes the existence of protomembranes with ancient lipids formed by Fischer–Tropsch synthesis. We determined the mesophase structure and fluidity of a prototypical decanoic (capric) acid-based system, a fatty acid with a chain length of 10 carbons, and a lipid system consisting of a 1:1 mixture of capric acid with a fatty alcohol of equal chain length (C10 mix). To shed light on the mesophase behavior and fluidity of these prebiotic model membranes, we employed Laurdan fluorescence spectroscopy, which reports on the lipid packing and fluidity of membranes, supplemented by small-angle neutron diffraction data. The data are compared with data of the corresponding phospholipid bilayer systems of the same chain length, 1,2-didecanoyl-sn-glycero-3-phosphocholine (DLPC). We demonstrate that the prebiotic model membranes capric acid and the C10 mix show formation of stable vesicular structures needed for cellular compartmentalization at low temperatures only, typically below 20 °C. They reveal the fluid-like lipid dynamic properties needed for optimal physiological function. High temperatures lead to the destabilization of the lipid vesicles and the formation of micellar structures.
Fichier principal
Vignette du fichier
2023 Misuraca Membranes-13-00386.pdf (1.49 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04297292 , version 1 (21-11-2023)

Identifiants

Citer

Loreto Misuraca, Roland Winter, Bruno Demé, Philippe M Oger, Judith Peters. Molecular Rearrangements in Protomembrane Models Probed by Laurdan Fluorescence. Membranes, 2023, 13 (4), pp.386. ⟨10.3390/membranes13040386⟩. ⟨hal-04297292⟩
6 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More