Article Dans Une Revue Nature Communications Année : 2023

Expulsion mechanism of the substrate-translocating subunit in ECF transporters

Chancievan Thangaratnarajah
Mark Nijland
  • Fonction : Auteur
Luís Borges-Araújo
Aike Jeucken
  • Fonction : Auteur
Jan Rheinberger
Siewert J Marrink
Cristina Paulino
Dirk J Slotboom

Résumé

Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF module) Previous data indicate that the S-component topples within the membrane to alternately expose the binding site to either side of the membrane. In many ECF transporters, the substrate-free S-component can be expelled from the ECF module. Here we study this enigmatic expulsion step by cryogenic electron microscopy and reveal that ATP induces a concave-to-convex shape change of two long helices in the motor, thereby destroying the S-component’s docking site and allowing for its dissociation. We show that adaptation of the membrane morphology to the conformational state of the motor may favour expulsion of the substrate-free S-component when ATP is bound and docking of the substrate-loaded S-component after hydrolysis. Our work provides a picture of bilayer-assisted chemo-mechanical coupling in the transport cycle of ECF transporters.

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hal-04296762 , version 1 (20-11-2023)

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Chancievan Thangaratnarajah, Mark Nijland, Luís Borges-Araújo, Aike Jeucken, Jan Rheinberger, et al.. Expulsion mechanism of the substrate-translocating subunit in ECF transporters. Nature Communications, 2023, 14 (1), pp.4484. ⟨10.1038/s41467-023-40266-1⟩. ⟨hal-04296762⟩
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