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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2023

Activation energy for pore opening in lipid membranes under an electric field

Résumé

The standard model of pore formation was introduced more than fifty years ago, and it has been since, despite some refinements, the cornerstone for interpreting experiments related to pores in membranes. A central prediction of the model concerning pore opening under an electric field is that the activation barrier for pore formation is lowered proportionally to the square of the electric potential. However, this has only been scarcely and inconclusively confronted to experiments. In this paper, we study the electropermeability of model lipid membranes composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) containing different fractions of POPC-OOH, the hydroperoxidized form of POPC, in the range 0 to 100 mol %. By measuring ion currents across a 50-μm-diameter black lipid membrane (BLM) with picoampere and millisecond resolution, we detect hydroperoxidation-induced changes to the intrinsic bilayer electropermeability and to the probability of opening angstrom-size or larger pores. Our results over the full range of lipid compositions show that the energy barrier to pore formation is lowered linearly by the absolute value of the electric field, in contradiction with the predictions of the standard model.
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Dates et versions

hal-04292666 , version 1 (21-11-2023)

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Eulalie Lafarge, Pierre Muller, André Schroder, Ekaterina Zaitseva, Jan Behrends, et al.. Activation energy for pore opening in lipid membranes under an electric field. Proceedings of the National Academy of Sciences of the United States of America, 2023, 120 (11), ⟨10.1073/pnas.2213112120⟩. ⟨hal-04292666⟩
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