The worldwide demand for renewable energy development is increasing rapidly, motivated by the challenge to reduce fossil fuel emissions in accordance with political imperatives to combat global climate change. Following the European Environmental Impact Assessment (EIA) Directive 85/337/EEC, several studies have been undertaken to identify the environmental conditions and ecosystem functioning at selected sites prior to OWF construction. However, these studies are generally focused on the conservation of some species and there is no holistic approach for analyzing the effects arising from OWF construction and operation. The goal of this study is to promote a sampling strategy to collect data on the different ecosystem compartments of the future Dieppe-Le Tréport (DLT) OWF site, adopting an ecosystem approach, which could be applied to other OWFs for the implementation of a trophic network analysis. For that, the collection of new data on biological compartments (phytoplankton, zooplankton, suprabenthos, meiofauna, benthos, demersal fishes, marine mammals and seabirds) are essential. Two cruises (winter: February 2022 and summer: September 2022) allowed to estimate the contribution of each zoological group in two main benthic habitats: coarse sand and medium clean sands, located on the site. In addition, isotopic analyses of the different biological compartments from phytoplankton to seabirds were also done as well as, stomach contents on demersal fish. These studies will offer a more integrated approach and will allow to characterize the trophic web functioning of the future OWF and in the vicinity of the OWF before the construction.