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Article Dans Une Revue Nature Biotechnology Année : 2023

Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity

Thomas Panier
Matthieu Piel

Résumé

Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5–9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins.
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Origine : Publication financée par une institution

Dates et versions

hal-04290181 , version 1 (22-11-2023)

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Lucie Ludvikova, Emma Simon, Mathieu Deygas, Thomas Panier, Marie-Aude Plamont, et al.. Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity. Nature Biotechnology, 2023, ⟨10.1038/s41587-023-01893-7⟩. ⟨hal-04290181⟩
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