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Poster De Conférence Année : 2023

Thermotolerant signaling induced by plant NLR TIR domains

Résumé

Plant immunity is threatened by global changes such as temperature rising. More particularly, immunity mediated by intracellular receptors of the NLR family is often inhibited under high temperature. Yet, mechanisms involved are poorly understood. Overexpressing TIR signaling modules isolated from NLRs (TIRs) is sufficient to activate an autoimmune response in absence of pathogens. By using inducible transgenic Arabidopsis lines expressing isolated TIRs or full-length NLRs, we showed that TIR-induced signaling is tolerant to high temperature (30°C), in contrast to full-length receptors. This discovery suggests that an increase in temperature affects NLR receptors but that downstream signaling remains intact and hence is thermotolerant. By using reverse genetics and q-PCR analyses, we showed that immune signaling mediated by several TIRs shares genetic components required for full-length TNLs signaling, and activates similar defense genes, which expression is maintained at 30°C. Interestingly, we showed that TIR signaling is not potentiated by PTI, in contrast to full-length NLRs. Thus, we hypothesize that in addition to sensing the presence of pathogen effectors, NLRs can also sense changes in their environment, such as a temperature increase or PTI-derived signals. This work opens perspectives to decipher whether naturally occurring TIR-containing truncated NLRs can also induce thermotolerant immunity and provide disease resistance resilient to a warming climate.
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Dates et versions

hal-04289521 , version 1 (16-11-2023)

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Héloïse Demont, Céline Remblière, Laurent Deslandes, Maud Bernoux, M. Bernoux. Thermotolerant signaling induced by plant NLR TIR domains. IS-molecular plant microbe interaction2023, Jul 2023, Providence (Rhode Island), United States. Zenodo, 2023, ⟨10.5281/zenodo.8366694⟩. ⟨hal-04289521⟩
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