Chronic kidney disease is a major medical problem, causing more than a million deaths each year in the world. Peripheral renal microvascular damage is found in most chronic kidney diseases, yet noninvasive and quantitative diagnostic tools are still lacking. Ultrasound Localization Microscopy (ULM) can assess tissue microvasculature with unprecedented resolution. In this work, we studied the feasibility of ULM in human kidney allografts with a standard low frame rate ultrasound scanner. The acquisition parameters were derived from Contrast-Enhanced Ultrasound (CEUS) examinations by increasing the duration of the recorded clip at the same plane. ULM images were compared with Color Doppler, Advanced Dynamic Flow (ADF), and Superb Microvascular Imaging (SMI) with a contrast agent. Despite some additional limitations due to movement and saturation artifacts, ULM identified vessels 2 to 4 times thinner compared with Doppler modes; i.e. the mean ULM smallest analyzable vessel cross section is 0.3 ± 0.2 mm in 7 patients. Additionally, ULM was able to provide quantitative information on blood velocities in the cortex area. In this proof-of-concept study, ULM was shown to be a promising imaging technique for the qualitative and quantitative assessment of microvessels.