Effects of vitamin D3 on human lung fibroblasts derived from patients with IPF
Résumé
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease. Currently, no treatment can stop the progression of IPF. Vitamin D3 (VD) reduces experimental in murine models prevents <i>in vitro</i> activation fibroblasts. Moreover, depletion VD may be associated with reduced survival patients In this context, we determined present study if prevent pro-fibrotic functions human fibroblasts (HLFs) isolated from IPF control HLFs were derived surgical biopsies collected or primary cancer, respectively. (10-100 nM) barely prevented TGF-β1-induced differentiation HLFs. At 100 nM, slightly expression marker α-smooth muscle actin had effect on fibronectin collagen-1 expression. contrast, nM strongly inhibited aerobic glycolytic metabolism induced by TGF-β1 (3-100 also potently basal PDGF-dependent proliferation Using gene silencing technology, demonstrated that such effects mediated vitamin D receptor. addition, altered cell cycle increasing percentage arrested G0/G1 phase downregulating various regulatory proteins. conclusion, our shows low concentrations These results suggest it might interesting to assess potential clinical benefits supplementation IPF, especially slowing function decline.