Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood–brain barrier? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Comparative Neurology Année : 2023

Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood–brain barrier?

Résumé

The blood-brain barrier (BBB) is a physical interface between the blood and brain parenchyma, playing key roles in homeostasis. In mammals, BBB established thanks to tight junctions cerebral endothelial cells, involving claudin, occludin, zonula occludens proteins. Estrogens have been documented modulate permeability. Interestingly, of zebrafish, estrogen-synthesizing activity strong due high expression Aromatase B protein, encoded by cyp19a1b gene, radial glial cells (neural stem cells). Given estrogens function, we investigated their impact on genes involved junctions. We treated zebrafish embryos adult males with 17β-estradiol observed an increased junction claudin 5 only. females, treatment nuclear estrogen receptor antagonist (ICI182,780 ) had no impact. telencephalic injuries performed decreased gene that was partially reversed 17β-estradiol. This further confirmed extravasation experiments Evans blue showing estrogenic limits leakage. also highlighted intimate links neural suggesting cholesterol peripheral steroids could be taken up used as precursors for synthesis cells. Together, our results show provides alternative model investigate role integrity, both constitutive regenerative physiological conditions. link described capillaries steroidogenic encourages use this understanding mechanisms which get into tissue neurogenic activity.

Dates et versions

hal-04285432 , version 1 (14-11-2023)

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Citer

Elisabeth Pellegrini, Danielle Fernezelian, Cassandra Malleret, Marie-Madeleine Gueguen, Jessica Patche-Firmin, et al.. Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood–brain barrier?. Journal of Comparative Neurology, 2023, 531 (17), pp.1828-1845. ⟨10.1002/cne.25543⟩. ⟨hal-04285432⟩
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