TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2023

TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell

Résumé

Xenopus egg extract is a powerful material to modify cultured cells fate and to induce cellular reprogramming in mammals. In this study, the response of goldfish fin cells to in vitro exposure to Xenopus egg extract, and subsequent culture, was studied using a cDNA microarray approach, gene ontology and KEGG pathways analyses, and qPCR validation. We observed that several actors of the TGFβ and Wnt/β-catenin signaling pathways, as well as some mesenchymal markers, were inhibited in treated cells, while several epithelial markers were upregulated. This was associated with morphological changes of the cells in culture, suggesting that egg extract drove cultured fin cells towards a mesenchymal-epithelial transition. This indicates that Xenopus egg extract treatment relieved some barriers of somatic reprogramming in fish cells. However, the lack of re-expression of pou2 and nanog pluripotency markers, the absence of DNA methylation remodeling of their promoter region, and the strong decrease in de novo lipid biosynthesis metabolism, indicate that reprogramming was only partial. The observed changes may render these treated cells more suitable for studies on in vivo reprogramming after somatic cell nuclear transfer. © 2023, The Author(s).
Fichier principal
Vignette du fichier
s41598-023-36354-3.pdf (5.41 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-04283845 , version 1 (14-11-2023)

Licence

Paternité

Identifiants

Citer

Nathalie Chenais, Aurélie Le Cam, Brigitte Guillet, Jean-Jacques Lareyre, Catherine Labbé. TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell. Scientific Reports, 2023, 13 (1), pp.9967. ⟨10.1038/s41598-023-36354-3⟩. ⟨hal-04283845⟩
46 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More