MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarker and drug - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Chemical Science Année : 2023

MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarker and drug

Résumé

COVID-19 has afflicted people's life worldwide. Interleukin-6 (IL-6) is an important COVID-19 biomarker in human body fluid that can be used as reference to monitor COVID-19 in real-time and therefore to reduce the risk of virus transmission. On the other hand, Oseltamivir is a potential COVID-19 curing drug, but its overuse easily leads to hazardous side effects, calling for its real time monitoring in body fluids. For these purposes, a new Yttrium Metal-Organic Framework (Y-MOF) has been synthesized, in which the 5-(4-(imidazol-1-yl)phenyl)isophthalic linker contains a large aromatic backbone capable of strongly interacting with DNA sequences through π-π stacking interactions, which is appealing to build a unique sensor based on DNA functionalized MOFs. The MOF/DNA sequence hybrid luminescent sensing platform presents excellent optical properties associated with a high Förster Resonance Energy Transfer (FRET) efficiency. Furthermore, to construct a dual emission sensing platform, a 5'-carboxylfluorescein (FAM) labeled DNA sequence (S2) with stem-loop structure that can specifically interact with IL-6 has been associated to the Y-MOF. The resulting Y-MOF@S2 exhibits an efficient ratiometric detection of IL-6 in human body fluid with extremely high Ksv value 4.3×10 8 M-1 and a low detection limit (LOD) of 70 pM.
Fichier principal
Vignette du fichier
67_ChemSci.pdf (1.21 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY - Paternité

Dates et versions

hal-04283079 , version 1 (13-11-2023)

Identifiants

Citer

Xinrui Wang, Gilles Clavier, Yan Zhang, Kamal Batra, Nanan Xiao, et al.. MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarker and drug. Chemical Science, 2023, 14 (20), pp.5386-5395. ⟨10.1039/D3SC00106G⟩. ⟨hal-04283079⟩
4 Consultations
5 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More