Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cancers Année : 2022

Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling

Trevor J Mathias
Julia A Ju
Rachel M Lee
Keyata N Thompson
  • Fonction : Auteur
Makenzy L Mull
  • Fonction : Auteur
David A Annis
  • Fonction : Auteur
Katarina T Chang
Eleanor C Ory
  • Fonction : Auteur
Megan B Stemberger
  • Fonction : Auteur
Takashi Hotta
Ryoma Ohi
  • Fonction : Auteur
Michele I Vitolo

Résumé

Post-translational modifications (PTMs) of the microtubule network impart differential functions across normal cell types and their cancerous counterparts. The removal of the C-terminal tyrosine of α-tubulin (deTyr-Tub) as performed by the tubulin carboxypeptidase (TCP) is of particular interest in breast epithelial and breast cancer cells. The recent discovery of the genetic identity of the TCP to be a vasohibin (VASH1/2) coupled with a small vasohibin-binding protein (SVBP) allows for the functional effect of this tubulin PTM to be directly tested for the first time. Our studies revealed the immortalized breast epithelial cell line MCF10A undergoes apoptosis following transfection with TCP constructs, but the addition of oncogenic KRas or Bcl-2/Bcl-xL overexpression prevents subsequent apoptotic induction in the MCF10A background. Functionally, an increase in deTyr-Tub via TCP transfection in MDA-MB-231 and Hs578t breast cancer cells leads to enhanced focal gelatin degradation. Given the elevated deTyr-Tub at invasive tumor fronts and the correlation with poor breast cancer survival, these new discoveries help clarify how the TCP synergizes with oncogene activation, increases focal gelatin degradation, and may correspond to increased tumor cell invasion. These connections could inform more specific microtubule-directed therapies to target deTyr-tubulin.
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hal-04282910 , version 1 (13-11-2023)

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Trevor J Mathias, Julia A Ju, Rachel M Lee, Keyata N Thompson, Makenzy L Mull, et al.. Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling. Cancers, 2022, 14 (7), pp.1707. ⟨10.3390/cancers14071707⟩. ⟨hal-04282910⟩

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