VASH1–SVBP and VASH2–SVBP generate different detyrosination profiles on microtubules - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue (Data Paper) Journal of Cell Biology Année : 2022

VASH1–SVBP and VASH2–SVBP generate different detyrosination profiles on microtubules

Sung Ryul Choi
Chadni Sanyal
Thorsten B Blum
Fatma Krichen
Eric Denarier
Carsten Janke
Maria M Magiera
Isabelle Arnal
Michel O Steinmetz
Marie-Jo Moutin

Résumé

The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1-SVBP and VASH2-SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1-SVBP and VASH2-SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2-SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1-SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N-and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2-SVBP on microtubules compared to VASH1-SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH-SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1-SVBP and VASH2-SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions. .
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Dates et versions

hal-04282103 , version 1 (13-11-2023)

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Citer

Sacnicte Ramirez-Rios, Sung Ryul Choi, Chadni Sanyal, Thorsten B Blum, Christophe Bosc, et al.. VASH1–SVBP and VASH2–SVBP generate different detyrosination profiles on microtubules. Journal of Cell Biology, 2022, 222 (2), ⟨10.1083/jcb.202205096⟩. ⟨hal-04282103⟩
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