Article Dans Une Revue Nucleic Acids Research Année : 2023

Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast

Résumé

Telomeres are nucleoprotein comple x es that protect the chromosome-ends from eliciting DNA repair while ensuring their complete duplication. Pot1 is a subunit of telomere capping complex that binds to the G-rich o v erhang and inhibits the activation of DNA damage c hec kpoints. In this study, w e e xplore ne w functions of fission y east Pot1 b y using a pot1-1 temperature sensitive mutant. We show that pot1 inactivation impairs telomere DNA replication resulting in the accumulation of ssDNA leading to the complete loss of telomeric DNA. Recruitment of Stn1 to telomeres, an auxiliary factor of DNA lagging strand synthesis, is reduced in pot1-1 mutants and o v ere xpression of Stn1 rescues loss of telomeres and cell viability at restrictive temperature. We propose that Pot1 pla y s a crucial function in telomere DNA replication by recruiting Stn1-Ten1 and Pol α-primase complex to telomeres via Tpz1, thus promoting lagging-strand DNA synthesis at stalled replication forks.

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hal-04281712 , version 1 (13-11-2023)

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Pâmela C Carvalho Borges, Chaïnez Bouabboune, Jose Miguel Escandell, Samah Matmati, Stéphane Coulon, et al.. Pot1 promotes telomere DNA replication via the Stn1-Ten1 complex in fission yeast. Nucleic Acids Research, In press, ⟨10.1093/nar/gkad1036⟩. ⟨hal-04281712⟩
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