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Chapitre D'ouvrage Année : 2011

Electrotransfer of Plasmid DNA

Résumé

Various vectors have been developed in the field of gene transfer. However, important unresolved problems persist such as secondary toxicity or low gene transfer efficiency of viral as well as nonviral vectors. Therefore, an efficient and safe method of DNA delivery needs to be found. DNA electrotransfer is a physical method that consists of the local application of electric pulses after the introduction of DNA into the extracellular medium. Electrotransfer has proven to be one of the most efficient and simple nonviral methods of delivery. Moreover, it may provide an important alternative technique in the field of cell and gene therapies. The present chapter focuses on questions related to the mechanism of DNA electrotransfer, i.e., the basic processes responsible for membrane electropermeabilization, interaction of plasmid DNA with the plasma membrane, and its transport through the cytoplasm and into the nucleus.
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Dates et versions

hal-04281264 , version 1 (12-11-2023)

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Jean-Michel Escoffre, Marie-Pierre Rols, David Dean. Electrotransfer of Plasmid DNA. Clinical Aspects of Electroporation, Springer New York, pp.145-157, 2011, ⟨10.1007/978-1-4419-8363-3_13⟩. ⟨hal-04281264⟩
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