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Article Dans Une Revue Applied Sciences Année : 2023

The high resolutive detection of TiO$_2$ nanoparticles in human corneocytes via TEM/NanoSIMS correlation

Morgane Janin
Anthony Delaune
David Gibouin
Fabien Delaroche
Benjamin Klaes
Auriane Etienne
Armelle Cabin-Flaman

Résumé

Titanium dioxide (TiO$_2$) nanoparticles (NPs) are the subject of numerous studies and controversies on the risks they could pose to the environment and human health. When in contact with biological tissues, NPs can sometimes be challenging to precisely localize within subcellular structures (typically around 0.1 µm) when they exist as isolated NPs, particularly when using the SIMS approach. Indeed, the chemical signals produced by isolated NPs are very low, so they can be confused with background signals. This was the motivation behind our development of a new strategy for correlating TEM/SIMS to detect TiO$_2$ NPs in close proximity to cutaneous corneocytes. For this purpose, we initially developed a new tool for TEM and SIMS image registration based on a non-rigid image-deformation-enabling image overlay. Combining SIMS and TEM data through this overlay enhances NP localization’s precision. Secondly, we developed an algorithm based on the statistical analysis of multiplane SIMS images to denoise them. As a result, background noise was reduced, illuminating the low yet specific signals from isolated NPs. Finally, this new correlative approach enables the precise 3D localization of isolated NPs within the analyzed volume. We consider this method a breakthrough for subcellular-scale NP localization.

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hal-04278876 , version 1 (02-04-2024)

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Morgane Janin, Anthony Delaune, David Gibouin, Fabien Delaroche, Benjamin Klaes, et al.. The high resolutive detection of TiO$_2$ nanoparticles in human corneocytes via TEM/NanoSIMS correlation. Applied Sciences, 2023, 13 (22), pp.12189. ⟨10.3390/app132212189⟩. ⟨hal-04278876⟩
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