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Article Dans Une Revue Science of the Total Environment Année : 2024

Enzymatic degradation of tetracycline by Trametes versicolor laccase in a fluidized bed reactor

Résumé

Laccase from Trametes Versicolor was successfully immobilized on gelatin beads by a crosslinking reaction with glutaraldehyde. Immobilized laccases showed better stability towards pH and temperature than free laccases. Moreover, the immobilized laccases retained a good relative activity of 85% after 20 days of storage at 4°C. The degradation of tetracycline (TC) was studied with immobilized enzymes in both batch and fluidized bed reactors (FBR). The average degradation rate (1.59 mg h-1 Uenzymes-1) estimated over 24 hours in the FBR was almost 5 times higher than in the stirred tank reactor. Maximum degradation rate achieved was 72 ± 1% with a circulation flow rate of 80 mL min-1 and addition of air at a flowrate of 15 mL min-1. Study of the stability of the active beads under reaction conditions, shows that 45% of the TC was degraded after 5 cycles of 24 hours each. The toxicity of the TC solution before and after treatment was also investigated with microtox assays.
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Dates et versions

hal-04276448 , version 1 (09-11-2023)

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Marine Harguindeguy, Celine Pochat-Bohatier, José Sanchez-Marcano, M-P. Belleville. Enzymatic degradation of tetracycline by Trametes versicolor laccase in a fluidized bed reactor. Science of the Total Environment, 2024, 907, pp.168152. ⟨10.1016/j.scitotenv.2023.168152⟩. ⟨hal-04276448⟩
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