Biological fractionation of lithium isotopes by cellular Na + /H + exchangers unravels fundamental transport mechanisms
Résumé
Lithium (Li) has a wide range of uses in science, medicine, and industry, but its isotopy is underexplored, except in nuclear science and in geoscience. 6 Li and 7 Li isotopic ratio exhibits the second largest variation on earth's surface and constitutes a widely used tool for reconstructing past oceans and climates. As large variations have been measured in mammalian organs, plants or marine species, and as 6 Li elicits stronger effects than natural Li ($95% 7 Li), a central issue is the identification and quantification of biological influence of Li isotopes distribution. We show that membrane ion channels and Na +-Li + /H + exchangers (NHEs) fractionate Li isotopes. This systematic 6 Li enrichment is driven by membrane potential for channels, and by intracellular pH for NHEs, where it displays cooperativity, a hallmark of dimeric transport. Evidencing that transport proteins discriminate between isotopes differing by one neutron opens new avenues for transport mechanisms, Li physiology, and paleoenvironments.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers éditeurs autorisés sur une archive ouverte |
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