HE EMERGENCE OF A NEW GENETIC LINEAGE (ST-9316) OF NEISSERIA MENINGITIDIS SGROUP W IN NORTH FRANCE - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2019

HE EMERGENCE OF A NEW GENETIC LINEAGE (ST-9316) OF NEISSERIA MENINGITIDIS SGROUP W IN NORTH FRANCE

Ala-Eddine Deghmane
Eva Hong
Muhamed-Kheir Taha

Résumé

Materials and Methods All invasive isolates that are received at the National Reference Centre are analyzed by whole genome sequencing (WGS, NextSeq 500, Illumina). The isolates are uploaded regularly to the PUBMLST and periodically analyzed using genome comparator tools. The SplitsTree4 (version 4.13.1) is used to visualise the resulting distance matrices as Neighbor-net networks. Results Serogroup W isolates are increasing in France since 2013 and most of the isolates belonged to the clonal complex ST-11 and were derivatives of the South American UK strain. However, between 2013 and 2018, 24 cases of IMD due to isolates belonging to an unusual sequence type, the ST-9316, were identified in France mainly in the north part of the country. The majority of cases belonged to group W but few were of groups B and C. These isolates do not belong to any already defined clonal complex (CC). The cases were distributed as follows: 1 case in 2013, 2 cases in 2015, 6 cases in 2016, 9 cases in 2017 and 6 in 2018. The index case of this ST-9316 cluster was identified in December 2013 in the Hauts-de-France from a 29 years old woman. Cases were distributed in all groups of age. Coregenome MLST clustered all the isolates belonging to the ST-9316 together and were distantly separated from the other isolates that differed by more than 1300 alleles. However, those isolates were closer to the isolates of the CC865. The W/ ST-9316 isolates shared several identical allelic profiles that were different from those of lineages of CC11 isolates. Most of the corresponding genes encode metabolic enzymes. However, few other genes involved in interactions with host cells such as genes encoding FetA, PorA, factor H binding protein (Fhbp). Interestingly, all the W/ST-9316, unlike the W/CC11 isolates, lacked the hmbR gene encoding the haemoglobin receptor that is involved in the acquisition of iron from haemoglobin. Conclusion Changing meningococcal epidemiology may occur rapidly and locally. Surveillance requires extensive and powerful approaches such as WGS to rapidly adapt preventive strategies.
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hal-04270198 , version 1 (03-11-2023)

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  • HAL Id : hal-04270198 , version 1

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Ala-Eddine Deghmane, Eva Hong, Muhamed-Kheir Taha. HE EMERGENCE OF A NEW GENETIC LINEAGE (ST-9316) OF NEISSERIA MENINGITIDIS SGROUP W IN NORTH FRANCE. 15th EMGM Congress, European Meningococcal and Haemophilus Disease, May 2019, Lisabon, Portugal. ⟨hal-04270198⟩
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