Biochemical data documenting variations in mucilage polysaccharides in a range of glycosyltransferase mutants - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue (Data Paper) Scientific Data Année : 2023

Biochemical data documenting variations in mucilage polysaccharides in a range of glycosyltransferase mutants

Résumé

During Arabidopsis seed coat development, copious amounts of mucilage polysaccharides are produced in the epidermal cells. When hydrated on imbibition, these polysaccharides expand and are released to encapsulate the seed as a two-layered hydrogel. Polysaccharides are synthesized from UDP-sugars by glycosyltransferases (GTs) and several GTs, with differing activities, have been identified that contribute to mucilage polysaccharide synthesis. How these GTs orchestrate production of the complex polysaccharides found in mucilage remains to be determined. In this study, we generated a range of multiple GT mutants using either CRISPR/Cas9 targeted mutation or genetic crosses of existing T-DNA insertion mutants. Four traits for mucilage amounts or macromolecular properties were examined for four replicate seed lots from 31 different GT mutant combinations. This data provides a valuable resource for future genetic, biochemical, structural, and functional studies of the roles and properties of polysaccharides present in Arabidopsis mucilage and the relative contributions of different GTs to mucilage production.

Mots clés

Fichier principal
Vignette du fichier
2023_Aoi_ScientificData.pdf (2.4 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04270084 , version 1 (03-11-2023)

Licence

Paternité

Identifiants

Citer

Yuki Aoi, Abdelilah Benamar, Luc Saulnier, Marie-Christine Ralet, Helen North. Biochemical data documenting variations in mucilage polysaccharides in a range of glycosyltransferase mutants. Scientific Data , 2023, 10 (1), pp.702. ⟨10.1038/s41597-023-02604-2⟩. ⟨hal-04270084⟩
106 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More