Involvement of nifA and glnB genes in expression of nitrogen fixation genes in Azospirillum brasilense - Archive ouverte HAL Accéder directement au contenu
Chapitre D'ouvrage Année : 1995

Involvement of nifA and glnB genes in expression of nitrogen fixation genes in Azospirillum brasilense

Florence Arsène

Résumé

Using promoter fusions to lacZ as reporter gene, regulation of nif gene expression was studied in Azospirillum brasilense Sp7, in free-living state and during association of Azospirillum brasilense with wheat. In both conditions, nif gene expression requires NifA and the glnB product. The same protocol was successfully used to show that auxotrophic mutants (glnA, trpC, trpD) were impaired in the colonisation efficiency as compared to the wild type, whereas a nifA-Tn5 mutant was not.
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Dates et versions

hal-04268324 , version 1 (02-11-2023)

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Citer

Florence Arsène, Claudine Elmerich. Involvement of nifA and glnB genes in expression of nitrogen fixation genes in Azospirillum brasilense. Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. Azospirillum VI and Related Microorganisms, 37, Springer Berlin Heidelberg, pp.91-96, 1995, 978-3-642-79908-2. ⟨10.1007/978-3-642-79906-8_7⟩. ⟨hal-04268324⟩

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