Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN
Résumé
The paper presents experimental results regarding the preparation and characterization of a novel screenprinted modified gold electrode through formation of a self-assembled monolayer involving mercaptoundecanoic acid (MUA), followed by the attachment of C-Reactive Protein (CRP), as a specific metal binding protein with a strong affinity to uranyl ions. The average grain size, roughness and profile height during the stepwise assembly of the layered functionalized electrode were determined by Atomic Force Microscopy. Raman spectroscopy has been also applied to get more evidence on the immobilization of uranyl ions onto the modified Au/MUA/CRP electrode. Comparative cyclic voltammograms of ferro/ferricyanide couple in buffer solution using bare Au, Au/MUA and Au/MUA/CRP electrodes showed the self-assembled monolayer formation and addition of stable thin protein layer. It was demonstrated that the Au/MUA/CRP electrode could be suitable for quantitative detection through EIS technique based on the proportional response of charge transfer resistance against uranyl ions concentration.
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