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Article Dans Une Revue Journal of Cell Biology Année : 2020

A direct role for SNX9 in the biogenesis of filopodia

Iris Jarsch
Jonathan Gadsby
Annalisa Nuccitelli
  • Fonction : Auteur
Ludovic Pilloux
Claire Mulvey
Ulrich Dobramysl
Charles Bradshaw
Tristan Vaughan
Jennifer Gallop

Résumé

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell–cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.
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Dates et versions

hal-04266044 , version 1 (31-10-2023)

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Paternité

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Iris Jarsch, Jonathan Gadsby, Annalisa Nuccitelli, Julia Mason, Hanae Shimo, et al.. A direct role for SNX9 in the biogenesis of filopodia. Journal of Cell Biology, 2020, 219 (4), ⟨10.1083/jcb.201909178⟩. ⟨hal-04266044⟩

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